![]() Based on the results of these studies, it appears that if samples are held below 10☌ and are not allowed to freeze, most surface water E. coli densities between time 0 and the 48-h holding time, regardless of method. During phase 3, 8 of 13 sites showed no significant differences in E. When evaluated by the Colilert method, five of seven sites showed no significant difference in E. During phase 2, five of seven sites showed no significant difference between time 0 and 24 h by membrane filtration (mFC/NA-MUG). During phase 1, when 4☌ samples were evaluated by Colilert or by placing a membrane onto mFC medium followed by transfer to nutrient agar containing 4-methylumbelliferyl-β- d-glucuronide (mFC/NA-MUG), three of four sites showed no significant differences throughout the 48-h study. coli samples were analyzed at time 0 and at 8, 24, 30, and 48 h after sample collection. ![]() coli densities in surface water, studies were conducted in three phases, encompassing 24 sites across the United States and four commonly used monitoring methods. To determine whether holding time and storage conditions had an effect on E. Escherichia coli is a routinely used microbiological indicator of water quality.
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